I had the same type of situation as TJones once. I was in middle school, and had a hamster. I got a cat while still owning the hamster, and both would be in my room at the same time. The cat would chase and kill other rodents, but not my hamster. I believe this is because when she came close to him (under my supervision) I would hiss at her, or push her away from my hamster, or pet them both at the same time so she seemed to know he was "important" to me and not to harm him. They were never allowed out at the same time unsupervised, but she never tried to get into the cage either.
One time my hamster escaped into my room while I was at school. My mother saw this and freaked out (she's afraid of hamsters) and took me home early. When I went to my room, my cat was in it, sniffing my hamster who was running along the wall. She didn't harm him. It was a miracle.
This isn't to say that other animals would react the same way. A puppy is a puppy, and playfully could harm your gliders. That being said, I do believe training works. A puppy who learns what a glider is -- and that it's not food -- should leave them alone, if trained properly.
I also believe crate training a puppy while you're trying to potty train them is a great thing to do. Other people don't like putting them in a crate, which I understand, but it's easier in my opinion to potty train them if they're in a crate while unsupervised, so they don't mess in the house.
Keeping the gliders in a blocked-off room, if you don't want to crate train, is probably the best option in my opinion.